Signaling through dynamic linkers as revealed by PKA
نویسندگان
چکیده
منابع مشابه
Signaling through dynamic linkers as revealed by PKA.
Protein kinase A (PKA) is a prototype of multidomain signaling proteins functioning as allosteric conformational switches. Allosteric transitions have been the subject of extensive structural and dynamic investigations focusing mainly on folded domains. However, the current understanding of the allosteric role of partially unstructured linkers flanking globular domains is limited. Here, we show...
متن کاملPKA: assembly of dynamic macromolecular signaling
Although we have learned a great deal about the protein kinase superfamily from our structure/function studies of the free PKA catalytic (C) subunit and the free cAMP-bound regulatory (R) subunits, we did not understand how the C-subunit was inhibited in the holoenzyme complexes, nor did we understand at the molecular level how the holoenzymes were activated by cAMP. There are four functionally...
متن کاملDynamics of signaling by PKA.
The catalytic and regulatory subunits of cAMP-dependent protein kinase (PKA) are highly dynamic signaling proteins. In its dissociated state the catalytic subunit opens and closes as it moves through its catalytic cycle. In this subunit, the core that is shared by all members of the protein kinase family is flanked by N- and C-terminal segments. Each are anchored firmly to the core by well-defi...
متن کاملRegulation of neuronal PKA signaling through AKAP targeting dynamics.
Central to organization of signaling pathways are scaffolding, anchoring and adaptor proteins that mediate localized assembly of multi-protein complexes containing receptors, second messenger-generating enzymes, kinases, phosphatases, and substrates. At the postsynaptic density (PSD) of excitatory synapses, AMPA (AMPAR) and NMDA (NMDAR) glutamate receptors are linked to signaling proteins, the ...
متن کاملcAMP enhances BMP2-signaling through PKA and MKP1-dependent mechanisms.
Recent studies suggest that the elevation of intracellular cyclic adenosine monophosphate (cAMP) and the activation of the protein kinase A regulate BMP-induced osteogenesis. However, the precise mechanisms underlying the enhancing effect of cAMP on BMP2 signaling were not completely revealed. In this study we investigated the effect of elevated cAMP level and PKA activation on the BMP2-induced...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2013
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1312644110